Fluorowatcher

Two-channel Fluorescent Imager (Cy3/Cy5) for Microarrays and 96-well Plates

Fluorowatcher is a compact and portable fluorescent microarray imager.
By default, it operates on two channels (Cy3/Cy5) and supports 96-well plates, 12×8 well strips, and microscope slides (via adapter). Imaging is performed from below, so plates must have a transparent bottom. A high-sensitivity CMOS camera delivers higher signal-to-noise at shorter exposure times, and fluorescence is filtered using built-in fluorescence filter.
The instrument features an automated plate ejection mechanism and a high-sensitivity CMOS camera for fast, high-contrast imaging. For field and point-of-care applications, it can be powered by a 20 V lithium-ion battery.
With its compact design, Fluorowatcher easily fits into an airline carry-on bag, ensuring maximum portability without compromising performance.

  • AI Array Finder — automatic microarray grid detection & alignment
  • Grid layout and region mapping
  • Automated image acquisition and positioning
  • Analyzing spot intensity (total/mean/median) with background correction
  • Qualitative and quantitative assays, including calibration curves (4/5PL)
  • Point-and-shoot imaging at specified locations
  • Export results to PDF, CSV and Excel
  • Control of camera exposure, XYZ stage, and channel switching
Fluorescence channels CY3, CY5
Vessels 96-well plate / 12×8 well strip / 4 microscope slides
Light source Laser
Lifetime of the light source >10 000 hours
Data interface (controls) USB 2.0
Camera 3 MP, CMOS
Data interface (camera) USB 3.0
Magnification >10x
Image formats PNG, TIFF, BMP or JPEG; 16/24/32-bit
Focus Manual (software-controlled)
Exposure Up to ~30 s (programmable)
Software Included
PC requirements CPU: Intel i7 (with AVX2 support)
RAM: 8 GB
Video card: Nvidia GTX 1050 Ti 4GB, or better (Capability only with Nvidia cards GeForce 10/16/20/30 series)
SSD: 256 GB
OS: Windows 10 (64 bit)
Size (W×D×H) 330 × 345 × 170 mm
Weight (without power supply) Not more than 8 kg
Power supply Input AC 100–240 V 50/60 Hz
Output DC 20–24 V, 2.5 A

Optional fluorescence channels

Fluorophore Excitation, nm Emission, nm
ALEXA 430 445 520
CY3 520 615
CY5 635 697

Fluorophore Excitation, nm Emission, nm
ALEXA 405 405 438
CY3 520 615
CY5 635 697

Fluorophore Excitation, nm Emission, nm
ALEXA 405 405 438
FITC / ALEXA 488 470 520
ALEXA 568 520 640

nm FWHM Application
435 48
438 28
440 46 Sirius Emission
447 65 DAPI Emission / BFP & GFP Emission
448 25
452 51
466 45
472 35
475 56
482.5 36 CFP Emission
494 25
500 29
510 25 Cy2 Emission
510 89 FURA2 Emission
512 30 Fluorescein Emission
520 41 GFP Emission
520 77 Alexa Fluor® 488 Emission/GFP Emission
525 18 Qdot® 525 Emission
525 51 GFP/FITC Emission
527 22 SpectrumGreen™ Emission
530 62 Thiazole Orange Emission
534 25
534.5 48 FITC Emission
540 56 FITC Emission
543 27 YFP Emission
549 21 Alexa Fluor® 532 Emission
550 100 Broadband Green Fluorescence
560 32
561 21
562 46
nm FWHM Application
572 33 SpectrumGold™ Emission
575 35
578 22 Cy3.5 Emission
585 40
586 26 SpectrumOrange™ Emission
591.5 49 Cy3 Emission / TRITC Emission
605 22 Qdot® 605 Emission
607 42 GFP Emission / RFP Emission
615 26 Alexa Fluor® 594 Emission / Cy3 Emission
620 60
623 30 Texas Red® Emission
624 46 Texas Red® Emission
625 25 Qdot® 625 Emission
628 38 SpectrumRed™ Emission
631 28
640 20
640.5 81 mCherry Emission
648 20 FITC Emission
655 24 Qdot® 655 Emission
655 47
661 26 TO-PRO®-3 Iodide Emission
676 36 Cy5 Emission
692 47 Cy5 Emission
697 91 Alexa Fluor® 680 Emission / Cy5.5™ Emission
716 47 Cy5 Emission
785 71 Superbright780