Fluorowatcher

Fluorescent Imager

Fluorowatcher is a compact and portable fluorescent imager designed for imaging fluorescent molecules (for example, protein biochips). The instrument by standard operates on 2 channels: CY3 and CY5. The instrument is able to work with 96-well plate, a 12 x 8 well strip, as well as microscopic glasses (placed in a special adapter). The device takes images from the below, so the wells must have a transparent bottom. The device is equipped with a highly sensitive CMOS camera capable of taking more sensitive images in a short exposure time, which saves operating time. Image filtering goes through fluorescent filters. The instrument has an automatic mechanism of vessel ejection outside the body. The instrument can operate with a 20V portable lithium- ion battery, which makes the device usable in the field studies or point-of-care. The compact design allows the device to be carried in the hand luggage of the aircraft.

  • Grid lay-outing
  • Analysing images by the total brightness of the spots
  • Qualitative/quantitative analysis of the images
  • Creating Qualitative/quantitative assays
  • Taking images in specified locations
  • Report exports to PDF, CSV, EXCEL
  • Control of camera exposure, XYZ kinematics and channels
Fluorescence channels CY3, CY5
Vessels 96 well plate/12×8 well strip/4 microscope slides
Light source Laser
Lifetime of the light source >10 000 hours
Data interface for unit controls USB 2.0
Camera 3 MP, CMOS
Data interface for camera control USB 3.0
Magnification >10x
Image formats png/bmp/tiff/jpg, 16/24/32 bit
Focus Manual, adjustable via PC
Exposure Controllable, up to ~30 sec.
Software Included
PC requirements CPU: Intel i7 (with AVX2 support)
RAM: 8 GB
Video card: Nvidia GTX 1050 Ti 4GB, or better (Capability only with Nvidia cards)
SSD: 256 GB
OS: Windows 10 (64 bit)
Size (W×D×H) 330 × 345 × 170 mm
Weight, w/o power supply Not more than 8 kg
Power supply Input AC 100–240 V 50/60 Hz
Output DC 20-24 V, 2.5A

Optional fluorescence channels

Fluorophore Excitation, nm Emission, nm
ALEXA 430 445 520
CY3 520 615
CY5 635 697

Fluorophore Excitation, nm Emission, nm
ALEXA 405 405 438
CY3 520 615
CY5 635 697

Fluorophore Excitation, nm Emission, nm
ALEXA 405 405 438
FITC / ALEXA 488 470 520
ALEXA 568 520 640

nm FWHM Application
435 48
438 28
440 46 Sirius Emission
447 65 DAPI Emission / BFP & GFP Emission
448 25
452 51
466 45
472 35
475 56
482.5 36 CFP Emission
494 25
500 29
510 25 Cy2 Emission
510 89 FURA2 Emission
512 30 Fluoroscein Emission
520 41 GFP Emission
520 77 Alexa Fluor® 488 Emission/GFP Emission
525 18 Qdot® 525 Emission
525 51 GPF/FITC Emission
527 22 SpectrumGreen™ Emission
530 62 Thiazole Orange Emission
534 25
534.5 48 FITC Emission
540 56 FITC Emission
543 27 YFP Emission
549 21 Alexa Fluor® 532 Emission
550 100 Broadband Green Fluorescence
560 32
561 21
562 46
nm FWHM Application
572 33 SpectrumGold™ Emission
575 35
578 22 Cy3.5 Emission
585 40
586 26 SpectrumOrange™ Emission
591.5 49 Cy3 Emission / TRITC Emission
605 22 Qdot® 605 Emission
607 42 GFP Emission / RFP Emission
615 26 Alexa Fluor® 594 Emission / Cy3 Emission
620 60
623 30 Texas Red® Emission
624 46 Texas Red® Emission
625 25 Qdot® 625 Emission
628 38 SpectrumRed™ Emission
631 28
640 20
640.5 81 mCherry Emission
648 20 FITC Emission
655 24 Qdot® 655 Emission
655 47
661 26 TO-PRO®-3 Iodide Emission
676 36 Cy5 Emission
692 47 Cy5 Emission
697 91 Alexa Fluor® 680 Emission / Cy5.5™ Emission
716 47 Cy5 Emission
785 71 Superbright780