BioMassGene-4 is a 4 vessel bioreactor that registers the growth kinetics of microbial (bacterial and yeast) and suspension cell culture (insect, mammalian or plant cells) by measuring the turbidity, as well as it registers the gene expression based on fluorescence signal detection of fluorescent marker proteins such as GFP, and its derivatives e.g. — RFP, CFP and YFP (Green/Red/Cyan/Yellow Fluorescent Proteins), etc. Both the turbidity and fluorescence detection are non-invasive, thus they are performed real-time and online.

Bioreactor allows to perform following studies:

• Scale-up optimization of biotechnological process:
  record the yield of product and producers
• Record the induction or repression processes of the regulatory regions of the genome at the level of protein synthesis
• Many other studies where comfortable real-time online concentration and fluorescence measure-ments are key

• A 4 vesselmodule for cell cultivation based on Reverse–Spin principle of mixing in 50 ml falcon type tubes (e.g. TPP) with the option of controlling the growth of cells in real time.

• Turbidity measurement system, (range equivalent to 10³ – 10¹¹cells/ml) using patented multiangle light scat-tering method

• Built in Peltier thermoblock for incubation of the samples for both heating and cooling above/below room temperature
• Module for fluorescence signal registration that can be used for intra and extra-cellular fluorescent protein detection
• Software for logging, processing and analyzing of the results