Combined Real-time Biomass Registration Device and Gene Expression Analyser by Fluorescence

BioMassGene-4 is a 4 vessel bioreactor that registers the growth kinetics of microbial (bacterial and yeast) and suspension cell culture (insect, mammalian or plant cells) by measuring the turbidity, as well as it registers the gene expression based on fluorescence signal detection of fluorescent marker proteins such as GFP, and its derivatives e.g. — RFP, CFP and YFP (Green/Red/Cyan/Yellow Fluorescent Proteins), etc. Both the turbidity and fluorescence detection are non-invasive, thus they are performed real-time and online.

Bioreactor allows to perform following studies:

  • Scale-up optimization of biotechnological process:
    record the yield of product and producers
  • Record the induction or repression processes of the regulatory regions of the genome at the level of protein synthesis
  • Many other studies where comfortable real-time online concentration and fluorescence measure-ments are key

  • A 4 vesselmodule for cell cultivation based on Reverse–Spin principle of mixing in 50 ml falcon type tubes (e.g. TPP) with the option of controlling the growth of cells in real time.
  • Turbidity measurement system, (range equivalent to 103 – 1011cells/ml) using patented multiangle light scat-tering method

  • Built in Peltier thermoblock for incubation of the samples for both heating and cooling above/below room temperature
  • Module for fluorescence signal registration that can be used for intra and extra-cellular fluorescent protein detection
  • Software for logging, processing and analyzing of the results
Type of measurements Multi angle light scattering and fluorescence
Measurement range
10-10 000 NTU (equivalent to 0-150 OD600nm 103-1011 cells/ml)
Fluorescence measurement Single channel
Available channels BFP, GFP, YFP, RFP, iRFP or equivalent
Temperature setting range +4 ... 70 °C
Temperature resolution 0.1
Average cooling speed from 25°C to 4°C 0.7 °C/min
Average cooling speed from 25°C to 37°C 0.7 °C/min
Mixing speed 0-2500 rpm
Mixing timer for reverse motion 0-60 sec
Measurement interval 2-60 min
Cultural media volume 10-50 ml
Dimensions (W×D×H) 680 × 520 × 250 mm
Weight 8 kg
Outlet/Inlet filters Yes
Power Input AC 100-240 V 50/60 Hz
Output DC 12 V